Table 2:
Procedure | Design Parameters |
---|---|
Inoculation of starter culture | Type of culture (suspension vs. solid), amount of inoculum, total volume of culture |
Growth of starter culture | Temperature, rotation speed, duration, illumination |
Sub-culturing | Number of flasks, volume of culture and media |
Harvesting of GVs | Composition of lysis buffer and duration of lysis, concentration of cells |
Purification | Selection of centrifugation speed, type of rotor, tube and syringe needle |
Storage | Storage temperature, buffer and type of vial/tube |