. Author manuscript; available in PMC: 2018 Oct 13.

Published in final edited form as: Nat Protoc. 2017 Sep 7;12(10):2050–2080. doi: 10.1038/nprot.2017.081

Table 2:

Experimental Parameters for GV Production, Purification and Storage

Procedure	Design Parameters
Inoculation of starter culture	Type of culture (suspension vs. solid), amount of inoculum, total volume of culture
Growth of starter culture	Temperature, rotation speed, duration, illumination
Sub-culturing	Number of flasks, volume of culture and media
Harvesting of GVs	Composition of lysis buffer and duration of lysis, concentration of cells
Purification	Selection of centrifugation speed, type of rotor, tube and syringe needle
Storage	Storage temperature, buffer and type of vial/tube