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. 2018 Oct 12;9:4242. doi: 10.1038/s41467-018-06562-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — An alignment of chromosome 3 in Sk-2 and Sk-3 to sensitive Neurospora intermedia, which retains the ancestral gene order, shows a series of large inversions in the region between the killer and resistance loci. Blue color shows collinear regions and red inverted regions. All inversions are different between Sk-2 and Sk-3 and are interspersed with gene-poor and repeat-rich inserted regions. The location of the Sk-2 killer locus us marked with an orange circle and the rsk (resistance) locus and the centromeres are marked with green and purple circles, respectively. Tracks in gray show genes; GC content; repeat content; cytosine methylation; and H3K9me3, H3K27me3, and H3K4me2 histone methylation. H2K9me3 is a mark for constitutive heterochromatin, which in Neurospora is highly associated with repeats; H3K27me3 is a mark for facultative heterochromatin, which is primarily found in subtelomeric regions; and H3K4me2 is a mark for euchromatic regions. Cytosine methylation and the three different histone modifications were estimated using three biological replicates, and here the sum of the triplicates are presented