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. 2018 Oct 12;8:15199. doi: 10.1038/s41598-018-33297-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Optical signal from Ilmol in response to action potentials. (A) Hippocampal neurons expressing Ilmol were imaged under current clamp. Top trace is a higher quality signal from a single trial recording at 1000 hertz yielding a 1.5% signal/action potential. Bottom trace is a lower quality signal giving a 1% signal per action potential. Corresponding neurons are shown on the right. Resting fluorescence is the grey scale image. Regions of interest generating traces on the left are shown in red. (B) Optical traces of Ilmol from different regions of a neuron (soma: red, processes: blue and yellow). The corresponding region of interest is shown on the right. Image on the left shows the changes in fluorescence during action potential. A frame subtraction image was generated by subtracting the average fluorescence of the three frames during the action potential (denoted by an asterisk) from the average fluorescence of 20 frames before the onset of action potentials (denoted by black bar below the yellow trace).