Table 2.
Physical properties of the arginine scan series of constructs.
Construct | State | Weighted τ (msec) | Fast τ (msec) | Slow τ (msec) | % fast |
---|---|---|---|---|---|
LK7_R1 | on | 8 ± 2 | 3 ± 1 | 17 ± 5 | 58 ± 15 |
off | 15 ± 2 | 2 ± 2 | 24 ± 5 | 37 ± 8 | |
LK7_R2 | on | 10 ± 3 | 4 ± 2 | 22 ± 5 | 64 ± 7 |
off | 10 ± 3 | 4 ± 1 | 32 ± 10 | 73 ± 6 | |
LK7_R3 | on | 14 ± 1 | 5 ± 1 | 24 ± 1 | 56 ± 3 |
off | 12 ± 1 | 5 ± 1 | 19 ± 2 | 52 ± 7 | |
LK7_R4 | on | 8 ± 1 | 3 ± 1 | 23 ± 2 | 72 ± 3 |
off | 8 ± 1 | 3 ± 1 | 28 ± 5 | 81 ± 3 | |
LK7_R5 | on | 10 ± 1 | 4 ± 1 | 29 ± 4 | 74 ± 2 |
off | 10 ± 2 | 3 ± 1 | 30 ± 10 | 68 ± 5 | |
LK7_R6 | on | 12 ± 2 | 4 ± 1 | 41 ± 5 | 79 ± 6 |
off | 9 ± 2 | 3 ± 1 | 24 ± 6 | 68 ± 5 | |
LK7_R7 | on | 11 ± 2 | 4 ± 1 | 30 ± 7 | 71 ± 4 |
off | 10 ± 1 | 4 ± 1 | 25 ± 4 | 70 ± 5 | |
LK11_R1 | on | 11 ± 1 | 4 ± 1 | 32 ± 6 | 73 ± 4 |
off | 15 ± 2 | 7 ± 1 | 29 ± 7 | 57 ± 10 | |
LK11_R2 | on | 9 ± 1 | 4 ± 1 | 30 ± 3 | 79 ± 3 |
off | 6 ± 1 | 3 ± 1 | 19 ± 3 | 80 ± 4 | |
LK11_R3 | on | 13 ± 1 | 4 ± 1 | 30 ± 3 | 66 ± 4 |
off | 15 ± 1 | 6 ± 1 | 26 ± 5 | 54 ± 8 | |
LK11_R4 | on | 10 ± 1 | 3 ± 1 | 23 ± 5 | 60 ± 10 |
off | 11 ± 1 | 3 ± 1 | 21 ± 3 | 54 ± 11 | |
LK11_R5 | on | 11 ± 1 | 4 ± 1 | 29 ± 4 | 72 ± 4 |
off | 12 ± 2 | 4 ± 1 | 33 ± 6 | 71 ± 3 | |
LK11_R6 | on | 8 ± 2 | 3 ± 1 | 23 ± 5 | 75 ± 4 |
off | 7 ± 1 | 3 ± 1 | 19 ± 3 | 73 ± 7 | |
LK11_R7 | on | 14 ± 2 | 5 ± 1 | 40 ± 8 | 72 ± 6 |
off | 10 ± 2 | 3 ± 1 | 28 ± 7 | 73 ± 4 | |
LK11_R8 | on | 7 ± 1 | 3 ± 1 | 22 ± 2 | 78 ± 2 |
off | 8 ± 1 | 3 ± 1 | 26 ± 3 | 80 ± 3 | |
LK11_R9 | on | 10 ± 1 | 4 ± 1 | 30 ± 3 | 76 ± 3 |
off | 7 ± 1 | 3 ± 1 | 19 ± 3 | 76 ± 2 | |
LK11_R10 | on | 11 ± 3 | 4 ± 1 | 28 ± 7 | 71 ± 3 |
off | 11 ± 2 | 4 ± 1 | 29 ± 2 | 73 ± 5 | |
LK7_R6_K159R | on | 11 ± 2 | 4 ± 1 | 29 ± 3 | 71 ± 5 |
off | 9 ± 2 | 3 ± 1 | 18 ± 2 | 63 ± 5 | |
LK11_R3_K159R | on | 10 ± 1 | 4 ± 1 | 26 ± 4 | 72 ± 5 |
off | 11 ± 1 | 6 ± 1 | 27 ± 4 | 74 ± 3 |