Fig. 2. CIRBP promotes BCa cells proliferation and migration in vitro, increases the phosphorylation state of ERK1/2 and p38, also promotes the EMT in BCa cells.

a Distinct BCa cells (UM-UC-3 and 5637) transfected with NC (red), siCIRBP (brown), vector (green) or CIRBP overexpression plasmid (blue) were allowed to grow at the indicated times, and Cell growth and viability were evaluated by MTT assay. b Clonogenic survival assay evaluated Colony formation, and statistical analysis of the clone number. c Cell migration was evaluated by transwell migration assay, and the relative cell number of migration was statistically analyzed. d Proteins in the MAPK family, including phosphorylated and total ERK1/2 and p38, were analyzed by Western blot, suggesting activation of ERK1/2 and p38 by CIRBP in BCa cells. e Western blot analyzed the proteins (E-cadherin, N-cadherin, Vimentin, β -catenin and snail) involved in EMT after 48 h transfection of siCIRBP in 5637 cells or CIRBP overexpression plasmid in UM-UC-3 cells. Means ± standard deviation from three independent experiments. Student t test was used for statistical analysis