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. 2018 Oct 12;9(10):1046. doi: 10.1038/s41419-018-1109-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a The green fluorescence of stable cell lines, and qRT-PCR analysis verification of CIRBP knockdown efficiency by lentiviral-CIRBP-shRNA. b Xenograft models (n = 4) were established by subcutaneously inoculating LV-NC cells or LV-shCIRBP cells and allowed to grow for 6 weeks, then the mice were sacrificed and the tumors were taken out and weighed. c tumor volume measurement and tumor weight. d Representative H&E staining and immunefluorescence staining of xenograft tumors from the tumor-bearing mice of the LV-NC group and LV-shCIRBP group, indicating the downregulation of CIRBP (red) in LV-shCIRBP group. Nuclei were stained by DAPI (blue). e Pulmonary metastasis models (n = 3) were established by tail intravenous injecting LV-shCIRBP UM-UC-3 cells or LV-NC UM-UC-3 cells, the fluorescence intensity of pulmonary metastasis tumor was measured to evaluate the migration capacity. Representative H&E staining of lung tissues indicating the pulmonary metastasis tumors (pointed by the arrows). Statistical analysis of the fluorescence intensity was calculated using T-test. Means ± standard deviation from three independent experiments