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. 2018 Oct 12;9(10):1046. doi: 10.1038/s41419-018-1109-5

Fig. 7. Overexpression of PTGIS suppresses BCa cells proliferation and migration in vitro, increases the protein level of PPARγ and suppresses EMT. PTGIS knockdown could partially rescue the inhibition of migration and proliferation in siCIRBP-treated BCa cells.

Fig. 7

a After transfection with PTGIS overexpression plasmid, cell proliferation was performed by MTT assay. b Clonogenic survival assay and statistical analysis of the clone number. c Transwell migration assay and the statistical analysis of relative cell number of migration. d, e Western blot analysis of PPARγ and EMT markers (E-cadherin, N-cadherin, Vimentin and β -catenin) after 48 h transfection of PTGIS overexpression plasmid. f Western blot analysis of CIRBP, HIF-1α and PTGIS expression in siCIRBP-treated cells as well as in cells over-expressing CIRBP. g, h Rescue experiment of siPTGIS: Transwell migration assay and MTT assay