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. 2018 Oct 12;8:15202. doi: 10.1038/s41598-018-33227-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Inhibition of the Eleanor RNA cloud, ESR1 mRNA, and LTED cell proliferation by the phytoalexin fractions. (A) The Eleanor RNA cloud regressed upon treatment with the biochemical fractions of the soybean extracts. LTED cells were treated with each phytoalexin fraction (Fig. 1) and subjected to RNA FISH to visualize Eleanor foci (green). The nucleus was counterstained with DAPI (blue). Resveratrol has been previously shown to inhibit Eleanors¹⁰. Scale bar, 10 µm. (B) Transcription of ESR1 mRNA was inhibited with the extract fractions. Quantitative RT-PCR was performed to measure relative ESR1 mRNA levels in LTED cells treated as indicated. Values were normalized against GAPDH mRNA, and values for cells treated with DMSO (control) were set to 1. The bars represent the means ± S.D. n > 3. Resveratrol has been previously shown to efficiently inhibit ESR1 mRNA¹⁰. ***p < 0.001; *****p < 0.00001. (C) LTED cell proliferation was inhibited by the extract fractions. LTED cells were treated as indicated, and the viabilities were measured. Resveratrol has been previously shown to inhibit LTED cell proliferation¹⁰ *****p < 0.00001.