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. 2018 Oct 12;8:15195. doi: 10.1038/s41598-018-33305-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Morphological characterization of the myofibroblasts isolated from porcine ileum. (A) Characteristics of porcine ileum myofibroblasts. Myofibroblasts could be observed at 5 days after isolation. They had a cobblestone like morphology. They could be further sub- passaged as a continuous cell line. (B) A third passage of myofibroblasts was identified by immunofluorescence staining. Cells stained characteristically for intestinal myofibroblasts. They were positive for α-smooth muscle actin, vimentin and fibronectin, while cells were negative for sucrase-isomaltase, cytokeratin and desmin. (C) Primary ileum epithelial cells were used as control and stained with all markers that were used for the staining of the myofibroblasts. Scale bar: 25 µm.