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. 2018 Oct 12;8:15195. doi: 10.1038/s41598-018-33305-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Evaluation of different methods to support the growth of primary porcine ileum and colon epithelial cells. (A) Porcine ileum epithelial cells were cultured without myofibroblasts on a porcine collagen type I/III coated plate. They could live approximately 2 days before detaching and dying, fibroblasts took over 6 days after seeding. (B) Porcine ileum epithelial cells were cultured without myofibroblasts on a porcine collagen type I/III coated plate with medium supplemented with 20% conditioned medium collected from ileum myofibroblasts. Epithelial cells could be maintained for less than 3 days and a few tiny epithelial clusters were present at 6 days post seeding. Porcine ileum (C) and colon (D) epithelial cells were cultured in the presence of ileum myofibroblasts. Epithelial cells grew into a monolayer 3 days post co-cultivation and maintained their morphology for more than 6 days.