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. 2018 Oct 12;8:15195. doi: 10.1038/s41598-018-33305-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Kinetics of rotavirus replication in primary ileum and colon epithelial cells co-cultured with myofibroblasts. Three days post co-cultivation, cells were inoculated with rotavirus RVA/pig-tc/BEL/RV277/1977/G1P[7] strain at an m.o.i. = 1. At different time points post inoculation, (A) infected cells were visualized by immunofluorescence staining (red represents cytokeratin and green represents rotaviral antigen positive cells), (B) the percentage of infected epithelial cells was determined, (C) intra- and extracellular virus titers were assessed and (D) intra- and extracellular viral RNA loads were quantified by RT-qPCR. Scale bar: 25 µm. Data are expressed as mean ± standard deviation of the results of 3 separate experiments. Statistically significant differences in comparison with the data from 0 h p.i. are represented as *P <0.05 or **P <0.01.