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. 2018 Oct 12;8:15195. doi: 10.1038/s41598-018-33305-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Susceptibility of porcine primary enterocytes to different rotavirus genotypes present in fecal suspension of diarrheic pigs. The infected cells were visualized by immunofluorescence staining at 24 h post inoculation (A). Viral RNA titer with supernatant of co-cultured primary porcine enterocytes inoculated with different rotavirus fecal suspensions at 0 h and 24 h post inoculation (B). Scale bar: 25 µm. Data are expressed as mean ± standard deviation of the results of 3 separate experiments. Statistically significant differences in comparison with the data from 0 h p.i. are represented as *P <0.05 or **P <0.01. <LOQ: below limit of quantification.