Figure 9.

Infection of ileum epithelial cells co-cultured with myofibroblasts with PEDVCV777. Twenty-four hours post isolation, cells were refreshed with culture medium with/without FBS. Three days post co-cultivation, cells were inoculated with PEDV CV777 Vero adapted strain containing 10^4.9 CCID50 or with fecal material containing 10⁷ viral RNA copies/ml of wild type CV777. Twenty-four hours post inoculation, the infection was visualized by immunofluorescence staining (A) and the total number of infected epithelial cells in a 24 well plate was determined (B). Scale bar: 10 µm. Data are expressed as mean ± standard deviation of the results of 3 separate experiments.