Fig. 2.
Biophysical analysis of the DC-34/MYC G4 interaction. a CD thermal melt of the MYC G4 Pu22 (10 µM) in the absence (blue) and presence (red) of DC-34 (40 µM). b Table of changes in thermal melting temperatures in the presence of four equivalents of DC-34 (measured by circular dichroism) and KD values of DC-34 against a panel of G4-forming sequences and dsDNA. c Fluorescence intensity curves of 5′ Alexa Fluor labeled Pu22 MYC upon titration of DC-34. KD = 9.4 ± 1.1 µM. d Sensorgrams corresponding to 15 to 30 µM injections of DC-34 over a 5′ biotin labeled MYC sensor chip. KD = 1.4 ± 1.2 µM. e Sequence of MYC Pu 27 and Pu22