Fig. 4.

Effects of DC-34 on gene expression. a qPCR analysis of the indicated G4 containing genes following 5 μM treatment in L363 cells. b qPCR analysis of G4-associated genes at 48 h as a function of [DC-34]. c qPCR analysis of G4-associated genes after 48 h treatment with Braco-19, a pan-G4 binder, D089, a MYC G4 analog, and DC-34 at the indicated doses in L363 cells. Data in a–c are the average log₂ values for ΔΔC_t of three replicates in L363 cells. d Effects of D089 (15 µM) and DC-34 (5 µM) on expression of genes (Nanostring) at the indicated time points. G4 controlled genes including MYC (red) are highlighted in color. Fitted linear regression lines (solid) for gene expression and identity lines (dashed) for drugs are indicated