Figure 5.

DNAM-1 on SPMs contributes to the GC reaction in mediastinal lymph nodes after immunization. (A,B) LPMs and SPMs were isolated from WT or Cd226^−/− donors, and 4 × 10⁴ cells were transferred i.p. into Cd226^−/− recipient mice. At the same time, the recipient mice were immunized i.p. with TNP–OVA in alum adjuvant. Seven days after immunization, mediastinal lymph nodes were collected; the cells were stained with GL7 mAb, PNA, and anti-B220 mAb, and the frequencies of GC B cells (defined as the percentages of GL-7⁺PNA⁺cells in B220⁺ B cells) were determined by flow cytometry. Representative plots of staining (B220⁺ gate) from SPM-transferred mice (A). The scatter plot shows data combined from four independent experiments (B) error bars indicate SEM. ^*P < 0.05. One outlier was omitted from Cd226^−/−.