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. 2018 Oct 12;9(10):1045. doi: 10.1038/s41419-018-1020-0

Fig. 2. Over-expression of miR-126 suppress the tumor cell proliferation and metastasis in vitro.

Fig. 2

Transfection of the scramble and miR-126 mimics into two HCC cell lines, Hep3B and SMMC-7721, increased the expression level of miR-126 in a dose-dependent manner (a). Cell viability assay of Hep3B (b) and SMMC-7721 (c) after transfection of scramble and miR-126 mimics compared with control cells. d Representative images of scratch assay of Hep-3b and SMMC-7721 in 0 h, 36 h, 48 h after transfection scramble and miR-126 mimics. Scale bars, 50 μm. e Transmembrane invasion assay of Hep-3b and SMMC-7721 after transfection of scramble and miR-126 mimics. Scale bars, 200 μm. f EDU staining assay of Hep-3b and SMMC7721 after transfection scramble and miR-126 mimics was performed. Scale bars, 50 μm. g Colony formation assay of Hep-3b and SMMC7721 after transfection scramble and miR-126 mimics was performed. Mean ± SD, *P < 0.05, unpaired Student’s t test or one-way ANNOVA followed by multiple t test