Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Oct 12;9:4237. doi: 10.1038/s41467-018-06617-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — HH signaling is essential for heart regeneration. a Histological examination of the regenerating newt heart showing a mitotic cardiomyocyte in the injured tissue. b, c Immunohistochemical (b) and quantitative analysis of the total EdU⁺ cells (c) in the regenerating newt heart. The white arrowheads indicate the proliferating cardiomyocytes within the myocardium at specified time periods following injury. The boxed regions are magnified in the lower panels. Quantitative analysis represents counts from four randomly selected fields at 20× magnification from four replicates at each time period. d Gene set enrichment analysis using Bootstrap tools from regenerating newt heart tissue at the designated time periods postinjury. The color scale indicates the scaled expression levels of signaling pathways following heart injury. e, f qPCR analysis for shh and ptc-1 transcripts during cardiac regeneration in the newt (n = 4). g Schematic (top) of experimental protocol and whole-mount images of the regenerating heart obtained from control- and CyA-treated newts at 7 dpi and 30 dpi (n = 8 for each group). The dotted line represents the injured region of the heart. h Masson Trichrome staining of the regenerating hearts from control- and CyA-treated newts at 7 dpi and 30 dpi (n = 8 for each group). The dotted line represents the injured region of the heart. i, j Immunohistochemical staining (i) and quantification (j) of Desmin⁺-EdU⁺ cardiomyocytes in the regenerating heart from control- and CyA-treated newts at the designated time periods following injury (n = 6). The dotted line in panel i represents the injured region of the heart and the tissue in the boxed region is magnified in I′ and I″ panels. White arrowheads indicate the EdU⁺-cardiomyocytes. Data are presented as mean ± SEM (*p < 0.05; **p < 0.01) (see also Supplementary Figs 1 and 2) and scale bars = 200 μm (panels a, b, h, i) and 500 μm (panel g). Statistical tests were done using two-tailed unpaired Student’s t-test and one-way ANOVA