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. 2018 Oct 12;8:15183. doi: 10.1038/s41598-018-33636-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phylogenetic Neighbor-Joining tree of HIV pol gene sequences of HIV infected wives of IDUs in Manipur. Phylogenetic analysis of HIV-1 protease (PR) and reverse transcriptase (RT) gene sequences. Phylogenetic trees were generated from the sequenced HIV-1 PR (a) and RT (b) genes together with the corresponding viral gene of reference. The tree was inferred using the Neighbor-Joining method. The optimal tree with the sum of branch length = 2.5 is shown. The tree was computed using the Kimura 2-parameter method and are in the units of the number of base substitutions per site. The rate variation among sites was modeled with a gamma distribution (shape parameter = 1). The phylogenetic trees analyses were conducted in MEGA7. Manipur sequences were indicated with solid red triangle, Drug resistance of the ART naïve and ART treatment experienced individuals were represented with solid circle and solid stars respectively.