Table 2.
List of membrane protein structures utilizing a crystallization chaperone.
| Membrane Protein | Chaperone | PDB code | Resolution (Å) | Reference |
|---|---|---|---|---|
| Covalent Chaperones | ||||
| A2A adenosine receptor (A2AR) | T4 Lysozyme | 3EML | 2.6 | [91] |
| A2AR | T4 Lysozyme | 3QAK | 2.7 | [23] |
| β2 adrenergic receptor (β2AR) | T4 Lysozyme | 2RH1 | 2.4 | [92] |
| β2AR-Gs protein complex | T4 Lysozyme | 3SN6 | 3.2 | [49] |
| Nanobody | ||||
| CXCR4 chemokine receptor | T4 Lysozyme | 3ODU | 2.5 | [93] |
| Dopamine D3 receptor | T4 Lysozyme | 3PBL | 2.9 | [94] |
| Histamine H1 receptor | T4 Lysozyme | 3RZE | 3.1 | [95] |
| Noncovalent Chaperones | ||||
| AcrB multidrug exporter | DARPin* | 2J8S | 2.5 | [51] |
| AdiC arginine:agmatine antiporter | Fab | 3NCY | 3.2 | [96] |
| ApcT amino acid transporter | Fab | 3GI9 | 2.5 | [97] |
| β2AR | Fab | 2R4R | 3.4 | [98] |
| β2AR-Gs protein complex | T4 Lysozyme | 3SN6 | 3.2 | [49] |
| Nanobody | ||||
| Cytochrome bc1 complex | Fv* | 1EZV | 2.3 | [65] |
| Cytochrome c oxidase | Fv* | 1AR1 | 2.7 | [64] |
| Disulfide bond formation protein B (DsbB) | Fab | 2ZUQ | 3.3 | [99] |
| KcsA K+ channel (open) | Fab | 1K4C | 2.0 | [68] |
| (closed) | Fab* | 3EFD | 3.8 | [50] |
| KvAP voltage-gated potassium channel | Fab | 1ORQ | 3.2 | [100] |
| Fv* | 2A0L | 3.9 | [67] | |
| Nitric oxide reductase | Fab | 3O0R | 2.7 | [101] |
| SecYE protein-conducting channel | Fab | 2ZJS | 3.2 | [102] |
*
Chaperone obtained by recombinant methods