Figure 4.

Depiction of the humanized Abcb1a gene locus. Shown are the sequenced region (6089 bp, black) and the downstream adjacent mouse sequence not included in the sequencing (light grey). The human ABCB1 CDS was fused into the TSS, which left the untranslated base pairs TSS -1 to -6 of exon 2 behind (green). ABCB1 CDS has been introduced with its 3′UTR and additional 45 bp of human genomic downstream sequence. At the 3′ transition towards the mouse genomic sequence, a loxP site has been introduced which is flanked by altogether 99 bp of unknown origin. The same is true for the 5′ situated loxP site, which is flanked by additional 37 bp, respectively, and inserted into intron 1 of mouse Abcb1a. The picture is not proportional to the genomic arrangement.