Supplementary Figure 2. Western blot analysis of ABCB1 expression.

Western blot against ABCB1 confirmed PET imaging findings in hABCB1 animals and shows drastically reduced protein expression of any ABCB1 variant in hABCB1 as well as hABCB1^–/– mice. Data are mean ± standard deviation. Statistical significance was determined by 1-way ANOVA with Tukey's Honest Significant Difference post-hoc test, significance level p<0.05, *** p<0.001.