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. 2018 Oct 12;18:976. doi: 10.1186/s12885-018-4850-3

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — An in vitro environment simulating high stromal levels of FN improved the migration ability of the ESCC cell lines, Eca-109 and TE-1. a Changes in morphology induced by culture with MSC-conditioned medium for 42 h. Magnification, × 200. (b, c) Wound-healing assays (b) and Transwell assays (c) showed that culture with MSC-conditioned medium increased EECC cell migration ability. Magnification, × 40. The line chart in (a) shows the distance cells migrated over time, and the photographs in (b) show cells that traversed the membrane (Eca-109, P = 0.001; TE-1, P < 0.001). Scale bar = 100 μm