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. 2018 Oct 11;6:106. doi: 10.1186/s40425-018-0403-1

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — The scheme of our study. We prospectively enrolled patients with advanced GC who were scheduled to receive RAM-containing chemotherapy from January 2016 to August 2016. We obtained pre- and post-treatment paired blood and primary tumor samples by endoscopic biopsy to assess immune profiles from 20 patients. Archived FFPE samples were used for analyses of genomic alterations, MMR status, and EBV status. Tumor samples from 7 and 18 patients were subject to IHC and flow cytometry, respectively