Table 1.

Total VFA, NH₃-N, and lactic acid concentrations, individual VFA proportions, and acetic acid-to-propionic acid ratio

Items	HAY period		CON period		SEM
	Rumen	Reticulum	Rumen	Reticulum
Total VFA, mmol/dL
08:00	12.21	8.44	9.91	9.47	0.83
14:00	9.05	9.48	11.60	11.07	0.70
20:00	8.94	9.66	9.70	9.18	0.40
Acetic acid, %
08:00	73.58a	75.39	61.12A	66.45	0.53
14:00	71.91a	73.98	57.23	60.87	1.22
20:00	70.52A	73.80	59.46A	63.11	1.36
Propionic acid, %
08:00	17.68	16.73	17.49A	15.21	0.95
14:00	18.42	17.47	16.72	15.28	1.23
20:00	19.22A	17.35	18.33A	16.90	1.10
Butyric acid, %
08:00	7.05	6.37b	17.07A	14.67	0.48
14:00	8.15Aa	7.27b	21.44	19.63	0.57
20:00	8.70	7.65	18.52A	16.73	0.59
Others, %
08:00	1.70	1.51B	4.33	3.67	0.27
14:00	1.51Aa	1.28B	4.62a	4.21	0.30
20:00	1.57A	1.20B	3.68a	3.26	0.14
A/P Ratio
08:00	4.16	4.52	3.58A	4.47	0.26
14:00	3.91	4.26	3.56	4.12	0.36
20:00	3.69A	4.27	3.31A	3.82	0.30
NH3-N, mg/dL
08:00	9.02	8.17	5.62	6.44	0.61
14:00	4.75	5.21	3.46	2.95	0.51
20:00	4.70	5.18	5.98	6.33	0.79
Lactic acid, g/L
08:00	0.018	0.028	0.014	0.015	0.006
14:00	0.017	0.016	0.015	0.012	0.004
20:00	0.010A	0.015	0.375	0.381	0.059

^A,B denotes significant difference (P < 0.05) between the rumen and reticulum at the same period

^a,b denotes significant difference (P < 0.05) between the HAY and CON periods at the same location