Table 2.
Relative abundance (% of total sequences) of the major bacterial phyla and genera
| Items | HAY Period | CON Period | SEM | P value | ||||
|---|---|---|---|---|---|---|---|---|
| Rumen (n = 4) | Reticulum (n = 4) | Rumen (n = 4) | Reticulum (n = 4) | Period (P) | Location (L) | P x L | ||
| Phylum | ||||||||
| Firmicutes | 37.80 | 39.18 | 62.85 | 60.79 | 5.54 | 0.002 | 0.962 | 0.706 |
| Bacteroidetes | 30.26 | 31.11 | 10.84 | 14.19 | 5.89 | 0.014 | 0.762 | 0.822 |
| Actinobacteria | 3.36 | 1.70 | 4.72 | 3.46 | 1.16 | 0.114 | 0.401 | 0.814 |
| Genus | ||||||||
| Prevotella | 27.20 | 27.15 | 5.29 | 8.56 | 4.84 | 0.006 | 0.766 | 0.746 |
| Ruminococcus | 3.56 | 4.09 | 19.96 | 14.97 | 3.28 | 0.019 | 0.626 | 0.541 |
| Clostridium | 3.76 | 3.59 | 13.37 | 14.22 | 2.16 | 0.006 | 0.910 | 0.839 |
| Butyrivibrio | 3.05 | 3.28 | 11.95 | 12.13 | 3.19 | 0.117 | 0.931 | 0.977 |
| Eubacterium | 3.24 | 3.75 | 1.41 | 1.98 | 0.34 | 0.025 | 0.027 | 0.912 |
| Oscillibacter | 3.84 | 3.40 | 0.34 | 0.49 | 0.46 | 0.001 | 0.825 | 0.608 |
| unclassified Clostridiales | 2.85 | 2.71 | 0.81 | 0.97 | 0.94 | 0.258 | 0.426 | 0.692 |
| Barnesiella | 0.88 | 1.22 | 3.25 | 3.97 | 1.11 | 0.077 | 0.743 | 0.879 |
| Acetivibrio | 1.89 | 1.66 | 1.11 | 1.12 | 0.87 | 0.716 | 0.459 | 0.996 |
| unclassified Lachnospiraceae | 1.95 | 2.01 | 0.28 | 0.30 | 0.59 | 0.173 | 0.593 | 0.431 |
| Succiniclasticum | 1.70 | 1.31 | 0.65 | 0.90 | 0.26 | 0.037 | 0.800 | 0.280 |
| Olsenella | 0.67a | 0.63b | 2.31ac | 1.57bc | 0.39 | 0.012 | 0.144 | 0.024 |
| unclassified Clostridiaceae | 0.95 | 1.09 | 0.96 | 1.53 | 0.31 | 0.800 | 0.265 | 0.552 |
| Bacteroides | 0.91 | 1.12 | 1.36 | 0.57 | 0.49 | 0.519 | 0.985 | 0.782 |
a-a, b-b, c-c means within a row, same superscripts differ significantly (P < 0.05)