Table 3.

Bacterial diversity calculated from 454 pyrosequencing data

Items	HAY Period		CON Period		SEM	P value
	Rumen (n = 4)	Reticulum (n = 4)	Rumen (n = 4)	Reticulum (n = 4)		Period (P)	Location (L)	P x L
OTUs	993	1115	582	632	106	< 0.001	0.587	0.604
ACE	2542	3057	1347	1669	234	< 0.001	0.242	0.563
Chao1	1774	2195	1003	1202	186	< 0.001	0.279	0.430
Shannon	5.60	5.85	4.49	4.50	0.28	< 0.001	0.613	0.455
Simpson	0.016	0.012	0.057	0.062	0.01	0.010	0.963	0.717