Table 4.
Copy number of mcrA and 16S rRNA genes identified form qRT-PCR
| Items | HAY Period | CON Period | SEM | P value | ||||
|---|---|---|---|---|---|---|---|---|
| Rumen (n = 4) | Reticulum (n = 4) | Rumen (n = 4) | Reticulum (n = 4) | Period (P) | Location (L) | P x L | ||
| Total methanogens | 5.78 | 5.34 | 4.73 | 3.97 | 0.23 | 0.004 | 0.018 | 0.583 |
| Fibrobacter succinogenes | 5.88 | 4.41 | 3.45 | 2.25 | 0.48 | < 0.001 | 0.119 | 0.600 |
| Megasphaera elsdenii | 2.97 | 2.89 | 3.76 | 5.99 | 0.64 | 0.180 | 0.741 | 0.230 |
| Streptococcus bovis | 7.00 | 6.74 | 4.89 | 7.62 | 0.55 | 0.465 | 0.279 | 0.279 |
| Ruminococcus albus | 6.13 | 4.65 | 4.76 | 4.45 | 0.37 | 0.068 | 0.123 | 0.147 |
| Ruminococcus flavefaciens | 7.60a | 6.03b | 5.87a | 6.42c | 0.45 | 0.270 | 0.582 | 0.019 |
| Selenomonas ruminantium | 7.20 | 6.65 | 6.21 | 6.11 | 0.24 | 0.041 | 0.135 | 0.481 |
a-a means within a row, same superscripts differ significantly (P < 0.05)