Figure 1. pUR4 attenuates fibronectin polymerization in unchallenged primary mouse cardiac fibroblasts 72 h post-treatment.
(A) FN immunofluorescence staining in healthy CF. Scale bars, 100 μm. (B) FN transcript level expression. n=5. (C) Cellular FN protein level expression (without matrix) (representative immunoblots, left, and densitometry, right). n=5. (D) Matrix FN deposition in the extracellular space of CF is attenuated with pUR4 (representative immunoblots, left, and densitometry, right). n=5. (E) FN is accumulated in the cultured media measured by ELISA. n=5. Statistical significance was determined with paired t-test: *P<0.05, **P<0.01. Data are represented as mean ± SEM.