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. 2018 Sep 18;108(4):784–792. doi: 10.1093/ajcn/nqy177

TABLE 2.

HPLC-MS/MS parameters for analysis of apocarotenoids from tomato juices and blood plasma1

Peak label Retention time, min Compound identity HPLC-APCI+-MS/MS transitions2 CE2 (eV) DP (V)
1 4.05 β-Apo-13-carotenone 259.2 > 175.13, 123.1, 119.1, 69.0 21, 27.5, 31, 27 60
2a, b 4.5, 4.6 Apo-13-lycopenone 259.2 > 175.1, 123.1, 119.1, 69.03 21, 27.5, 31, 27 60
3 4.75 β-Apo-15′-carotenal (retinal) 285.2 > 161.13, 123.1, 119.1, 69.0 15, 27.5, 15, 20 60
4 5.05 Apo-15′-lycopenal (acycloretinal) 285.2 > 161.1, 123.1, 119.1, 69.04 15, 27.5, 15, 20 60
5a, b 5.2, 5.35 β-Apo-14′-carotenal 311.2 > 159.13, 123.1, 95.1, 69.0 15, 27.5, 27, 27 60
—4 6.3 Apo-14′-lycopenal 311.2 > 159.13, 123.1, 95.1, 69.0 15, 27.5, 27, 27 60
6a, b 6.1, 6.4 β-Apo-12′-carotenal 351.2 > 161.13, 123.1, 119.1, 69.0 27, 27.5, 27, 27 80
7 6.7 β-Apo-10′-carotenal 377.3 > 123.1, 119.13, 95.1, 69.0 27.5, 27, 27, 27 80
8 7.2 Apo-12′-lycopenal 351.2 > 161.1, 123.1, 119.1, 69.03 27, 27.5, 27, 27 80
—4 7.8 Apo-10′-lycopenal 377.3 > 123.1, 119.1, 95.1, 69.03 27.5, 27, 27, 27 80
9 7.75 β-Apo-8′-carotenal 417.3 > 123.1, 119.13, 95.1, 69.0 27.5, 37, 37, 37 80
10 8.85 Apo-8′-lycopenal 417.3 > 123.1, 119.1, 95.1, 69.03 27.5, 37, 37, 37 80
11 9.3 Apo-6′-lycopenal 443.3 > 157.13, 105.03, 69.03 45, 60, 55 80
1

CE, collision energy; DP: declustering potential; MRM, multiple reaction monitoring; MS/MS, tandem mass spectrometry.

2

MRM experiments were scheduled to maximize dwell times for each apocarotenoid.

3

Quantifier ion; other MRMs are qualifier ions. The sum of the three MRMs was used for apo-6′-lycopenal.

4

Apo-10′- and -14′-lycopenal are not in the chromatogram because of the lack of authentic standards. Their retention times were determined from tomato samples as these analytes were absent in blood plasma. All analytes were run with the following parameters: entrance potential: 10 V; collision cell exit potential: 11 V; curtain gas: 45 psi; and source temperature: 400°C.