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. 2018 Sep 30;2018:2070971. doi: 10.1155/2018/2070971

Figure 3.

Figure 3

Hydrogen sulfide inhibited ROS-induced intracellular [Ca2+]i in 16HBE14o- cells. Logarithmic growth cells were incubated with calcium ion fluorescence probe Fura-2/AM and the F340/380 ratio value was detected by laser scanning confocal microscope. (a) The effect of CoCl2 on intracellular [Ca2+]i in different doses (0, 50, 100, 200, 400, or 1000 μmol/L). (b) NAC (1 mM, scavenger of ROS) inhibited the increase in F340/380 ratio induced by hypoxia (CoCl2, 400 μmol/L). (c) Effects of hydrogen sulfide on [Ca2+]i in different doses (0, 50, 100, 200, 300, 600, or 1000 μmol/L). (d) Effects of hydrogen sulfide (NaHS, 300 μmol/L) on [Ca2+]i induced by hypoxia (CoCl2, 400 μmol/L). The results were from 3 independent experiments (NS, ∗p < 0.05, ∗∗p < 0.01,∗∗∗p < 0.001 versus control group, #p < 0.05 versus hypoxia group, n=3).