Figure 5.
The effects of hydrogen sulfide on hypoxia-induced apoptosis. Exponential growth cells were stained with Hoechst 33342. The nuclei in control, NAC (1 mM), and NaHS (300 μM) groups have the normal phenotype demonstrating bright colors and homogeneity. The apoptotic nuclei emitted bright fluorescence and condensed. The number of apoptotic nuclei was significantly increased by CoCl2 (400 μM) treatment. NAC and NaHS significantly decreased the apoptosis of 16HBE14o- cells (NS, ∗∗p < 0.01, ∗∗∗p < 0.001 versus control group, ##p < 0.01, ###p < 0.001 versus hypoxia group, n=3).