Table 3. Cytotoxicity of the extracts of F. deltoidea leaves against WRL68 cells.
Sample | Extract/fraction | Incubation | ||
---|---|---|---|---|
24 hr (IC50 - µg/ml) | 48 hr (IC50 - µg/ml) | 72 hr (IC50 - µg/ml) | ||
FDS | Crude | N/D | N/D | N/D |
Water fraction | N/D | N/D | N/D | |
Ethyl acetate fraction | N/D | N/D | N/D | |
FDM | Crude | N/D | N/D | N/D |
Water fraction | N/D | N/D | N/D | |
Ethyl acetate fraction | N/D | N/D | N/D | |
FDB | Crude | N/D | N/D | 347.67 ± 2.52 |
Water fraction | 378.3 ± 5.51 | 306.7 ± 7.37 | 224 ± 21.17 | |
Ethyl acetate fraction | N/D | N/D | N/D |
Notes.
The experiment was conducted in a 96-well plate, each in triplicate. Cells were allowed to attach for 24 h after seeding. WRL68 cells were treated with various concentrations of the extracts of F.deltoidea crude extracts and fractions for 24, 48 and 72 h.
Results were expressed as means ± S.D. (n = 3).
IC50 = concentration of plant extracts (μ g/ml) that inhibited 50% of the cells.
N/D, no inhibition detected