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. Author manuscript; available in PMC: 2018 Oct 14.
Published in final edited form as: Curr Cancer Drug Targets. 2018;18(2):153–161. doi: 10.2174/1568009617666170222123925

Figure 4. Novel system for adenovirus vector production.

Figure 4

(A) Structure of Ad library with random 7 amino acid in the AB-loop of fiber-knob region. (B) High- diversity Ab library was produced with the newly developed rescue virus system by CRE-lox recombination in CRE-expressing, fiber-transcomplementing cell line (293CRE-69cell). The shuttle plasmid coding fiber library has a single loxP site in the upstream of the fiber gene. The produced library viruses were harvested 48 hours after shuttle plasmid transfection and rescue virus infection. (C)the strategy of replication-based Ad library screening [43].