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Probing 4F-Aβ40 interactions by tryptophan fluorescence quenching. Monitoring tryptophan (Trp2 residue of the 4F peptide) fluorescence quenching in (a) 50 μM 4F peptide solution and (b) ND1 containing 50 μM 4F peptides in presence (red and green traces) and absence (dashed black traces) of 25 μM Aβ40 at 37 °C at the indicated time intervals (0 min in dashed lines, 20 min in red, and 40 min in green).
