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. 2018 Oct 8;9:419. doi: 10.3389/fgene.2018.00419

FIGURE 2.

FIGURE 2

hGMSCs differentiation ability. (A1) Osteogenic differentiation of hGMSCs stained with Alizarin red S solution. (A2) RT-PCR of osteogenic related markers, RUNX2 and ALP. (B1) Adipogenic differentiation of hGMSCs stained with Oil Red to highlight cytoplasmic lipid droplets. (B2) RT-PCR of adipogenic related markers, PPARγ and FABP4. (C1) Chondrogenic differentiation of hGMSCs stained with Alcian blue solution. (C2) RT-PCR of chondrogenic related markers, ACAN and COL2A1 after 28 days of induction. (D1) Neurogenic differentiation was assessed by labeling with green-fluorescent marker, βIII-tubulin, cytoskeleton actin in red and nuclei in blue. (D2) RT-PCR of neurogenic related markers, Nestin and Enolase after 10 days of induction. Scale bar = 10 μm. ∗∗p < 0.01.