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. 2018 Oct 8;9:419. doi: 10.3389/fgene.2018.00419

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Copyright © 2018 Diomede, Zini, Pizzicannella, Merciaro, Pizzicannella, D’Orazio, Piattelli and Trubiani.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Light and transmission electron microscopy of EB-hGMSCs centre. (A) Light microscopy of semithin section stained with toluidine blue at high magnification. Bar: 10 μm. (B–F) Transmission electron microscopy. (B,C) Magnifications of areas similar to that shown in (A) shows ellipsoid (B) and irregular contour cells (C) with euchromatic nuclei (N), the cells are immersed in extracellular matrix (ECM). Bars: 2 μm. (D) At higher magnification, filaments (arrows), lipid droplets (L), mitochondria (M), vesicles (V), and rough endoplasmic reticulum (RER) are visible in the cytoplasm. Bar: 0.5 μm. (E) A junctional complex is present between two cells (arrow). Bar: 0.2 μm. (F) Higher magnification of extracellular matrix (ECM). Bar: 0.2 μm.