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. 2018 Oct 8;9:2324. doi: 10.3389/fmicb.2018.02324

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Copyright © 2018 Hossain, Ohsaki, Honda and Ueda.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression dynamics of KSHV lytic replication-related proteins. The KSHV lytic replication in wild-type (BC3), control (BC3-Scrambled^KO), and stable PML-knockout cells (BC3-PML^KO#1 and BC3-PML^KO#2) was induced with the treatment of TPA (25 ng/mL) and NaB (0.3 mM) and the cells were collected at the indicated time points. The total proteins were extracted and immunoblotted with the antibodies against the indicated specific proteins. β-tubulin was used as a loading control. The experiment was performed at least three times independently and one representative result is shown.