FIGURE 5.

Kaposi’s sarcoma-associated herpesvirus lytic DNA replication, virion production, and late-gene expression kinetics in PML-knockout cells. KHSV lytic replication was induced with TPA (25 ng/mL) and NaB (0.6 mM) treatment and the cells and the supernatants were collected at the indicated time points. The total DNA from the cells and KSHV virion DNA from the concentrated culture supernatants were extracted and the viral DNA was quantified as described in the Materials and Methods. (A) Intracellular KSHV DNA replication normalized by an endogenous control gene GAPDH. The data were shown as fold induction where the KHSV genomic DNA copy at the time zero was set at 1. (B) Extracellular virion production. The data were presented as an average of three replicates with error bars representing SEs. ^∗Statistically significant; P < 0.05. (C) Total proteins were extracted from the induced cells and immunoblotted with the antibodies against the indicated specific protein. β-tubulin was used as a loading control. (D) Graphs show relative expression levels of K8.1 and gB to β-tubulin generated from the Western blot band intensities.