FIGURE 6.

Overexpression of PML in BC3 cells. Wild-type BC3 cells were transduced with a halo-tagged PML-encoding retrovirus, and stable PML-expressing cells were established after hygromycin selection. (A) Total protein from the cells were extracted and immunoblotted with an anti (α)-Halo and an α-PML Ab for detection of PML. β-tubulin was used as a loading control. (B) Cells stably expressing PML were fixed and stained with a mouse α-Halo and a rabbit α-PML Ab and signals were visualized via immunofluorescence using an Alexa Fluor^® 488 (green) conjugated anti-mouse IgG and an Alexa Fluor^® 548 (red) conjugated anti-rabbit IgG, respectively. The cell nuclei were stained with DAPI (blue).