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. 2018 Oct 8;9:2181. doi: 10.3389/fmicb.2018.02181

FIGURE 1.

FIGURE 1

Cell permeability is increased in ectocervical and endocervical cells after exposure to L. iners and G. vaginalis but not L. crispatus bacteria-free supernatants. Cell permeability was measured in ectocervical cells (A) and endocervical cells (B) after a 48 h exposure to bacteria-free supernatants (10% v/v) from L. crispatus, L. iners, and G. vaginalis compared to non-treated control cells. Bacterial growth media alone acted as a negative control for the three bacteria-free supernatants tested. Cell permeability is expressed as fluorescence OD measurements from a fluorescent plate reader and is indicative of the movement of FITC-dextran from the top to the bottom insert of a transwell chamber system. Values are mean ± SEM. Asterisks over the individual bars represent comparisons to control; asterisks over solid lines represent comparisons between treatment groups. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.