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. 2018 Oct 8;9:2181. doi: 10.3389/fmicb.2018.02181

FIGURE 6.

FIGURE 6

Lactobacillus crispatus bacteria-free supernatants mitigate the G. vaginalis-induced miRNA expression profile in ectocervical cells. miRNA expression (A–G) was measured by QPCR in ectocervical cells after exposure to L. crispatus supernatants (5% v/v) on day 1 followed by G. vaginalis supernatants (5% v/v) on day 2 or vice versa for 24–48 h. Bacterial growth media alone acted as a negative control for the three bacteria-free supernatants tested. The ΔΔCT method was used for relative expression quantification using the endogenous reference gene RNU6B. Values are mean ± SEM. Asterisks over the individual bars represent comparisons to control; asterisks over solid lines represent comparisons between treatment groups. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.