Table 1.

Primer sequences for real-time quantitative PCR analysis of the expression of ALP, OCN genes, Collagen I, Runx2, Dlx5, Osterix and Smad1

Gene	Accession no	Primers (F=forward; R=reverse)
Akp2 (ALP)	NM_007431	F: 5′-TGCCTACTTGTGTGGCGTGAA-3′;
		R: 5′-TCACCCGAGTGGTAGTCACAATG-3′
OCN	NM_007541	F: 5′-AGCAGCTTGGCCCAGACCTA-3′;
		R: 5′-TAGCGCCGGAGTCTGTTCACTAC-3′
Collagen I	NM_007742	F: 5′-ATGCCGCGACCTCAAGATG-3′;
		R: 5′-TGAGGCACAGACGGCTGAGTA-3′
Runx2	NM_009820	F: 5′-CACTGGCGGTGCAACAAGA-3′;
		R: 5′-TTTCATAACAGCGGAGGCATTTC-3′
Dlx5	NM_010056	F: 5′-GACGCAAACACAGGTGAAA-3′;
		R: 5′-CGAGTTACACGCCATAGGG-3′
Osterix	AY_803733	F: 5′-TTTCTGCGGCAAGAGGTTCA-3′;
		R: 5′-TGCTCAAGTGGTCGCTTCTG-3′
Smad1	XM_006530746	F: 5′-GAGATGTTCAGGCAGTTGCT-3′;
		R: 5′-CTTCACCCACACGGTTGTT-3′
β-Actin	NM_007393	F: 5′-AGGAGCAATGATCTTGATCTT-3′;
		R: 5′-TGCCAACACAGTGCTGTCT-3′