TABLE 4.
Subcortical brain tissue mRNA expression at the end of the 32-wk diet treatment1
| P | |||||||
|---|---|---|---|---|---|---|---|
| Gene | 10% Lard | 10% Fish oil | 41% Lard | 41% Fish oil | Type of fat | Percentage of fat | Interaction |
| Mfsd2a | |||||||
| ΔΔCt | 0.000 ± 0.235 | 0.030 ± 0.205 | 0.792 ± 0.284 | 0.167 ± 0.172 | 0.1936 | 0.0454¥ | 0.1535 |
| 2(–ΔΔCt) | 1.000 (0.695, 1.439) | 0.896 (0.716, 1.340) | 0.578 (0.323, 0.901) | 0.891 (0.685, 1.159) | |||
| Glut1 | |||||||
| ΔΔCt | 0.000 ± 0.179 | –0.307 ± 0.145 | –0.040 ± 0.167 | –0.063 ± 0.147 | 0.3095 | 0.5280 | 0.3802 |
| 2(–ΔΔCt) | 1.000 (0.761, 1.314) | 1.238 (0.992, 1.544) | 1.028 (0.791, 1.336) | 1.045 (0.835, 1.308) | |||
| Pparα | |||||||
| ΔΔCt | 0.000 ± 0.194 | –0.564 ± 0.206 | –0.089 ± 0.259 | –0.261 ± 0.113 | 0.0687 | 0.5906 | 0.3271 |
| 2(–ΔΔCt) | 1.000 (0.744, 1.344) | 1.478 (1.079, 2.024) | 1.063 (0.713, 1.587) | 1.199 (1.009, 1.423) | |||
| Pparγ | |||||||
| ΔΔCt | 0.000 ± 0.080 | –0.004 ± 0.061 | –0.184 ± 0.077 | –0.074 ± 0.071 | 0.4668 | 0.0875 | 0.4372 |
| 2(–ΔΔCt) | 1.000 (0.880, 1.137) | 1.003 (0.914, 1.100) | 1.136 (1.008, 1.280) | 1.052 (0.944, 1.173) | |||
| Insr | |||||||
| ΔΔCt | 0.000 ± 0.133 | –0.206 ± 0.128 | –0.228 ± 0.170 | –0.148 ± 0.125 | 0.6557 | 0.5493 | 0.3129 |
| 2(–ΔΔCt) | 1.000 (0.811, 1.232) | 1.154 (0.948, 1.404) | 1.171 (0.901, 1.523) | 1.108 (0.915, 1.341) | |||
| Lrp1 | |||||||
| ΔΔCt | 0.000 ± 0.163 | –0.080 ± 0.127 | –0.177 ± 0.185 | –0.039 ± 0.149 | 0.8508 | 0.6654 | 0.4891 |
| 2(–ΔΔCt) | 1.000 (0.774, 1.292) | 1.057 (0.870, 1.283) | 1.131 (0.850, 1.505) | 1.027 (0.818, 1.290) | |||
| Abca1 | |||||||
| ΔΔCt | 0.000 ± 0.135 | –0.132 ± 0.085 | 0.143 ± 0.091 | 0.150 ± 0.119 | 0.5678 | 0.0560 | 0.5201 |
| 2(–ΔΔCt) | 1.000 (0.809, 1.237) | 1.096 (0.963, 1.248) | 0.906 (0.787, 1.042) | 0.901 (0.752, 1.080) | |||
| Cd36 | |||||||
| ΔΔCt | 0.000 ± 0.205 | –0.025 ± 0.128 | 0.009 ± 0.166 | 0.086 ± 0.172 | 0.8766 | 0.7234 | 0.7614 |
| 2(–ΔΔCt) | 1.000 (0.729, 1.372) | 1.018 (0.837, 1.237) | 0.994 (0.770, 1.284) | 0.942 (0.724, 1.225) | |||
| Apoe | |||||||
| ΔΔCt | 0.000 ± 0.106 | –0.039 ± 0.079 | –0.081 ± 0.102 | 0.154 ± 0.103 | 0.3232 | 0.5702 | 0.1687 |
| 2(–ΔΔCt) | 1.000 (0.847, 1.181) | 1.028 (0.911, 1.159) | 1.058 (0.903, 1.239) | 0.899 (0.769, 1.052) | |||
| App | |||||||
| ΔΔCt | 0.000 ± 0.088 | 0.142 ± 0.055 | 0.059 ± 0.094 | 0.193 ± 0.069 | 0.0807 | 0.4773 | 0.9568 |
| 2(–ΔΔCt) | 1.000 (0.873, 1.146) | 0.906 (0.833, 0.986) | 0.960 (0.830, 1.109) | 0.875 (0.788, 0.971) | |||
| Bdnf | |||||||
| ΔΔCt | 0.000 ± 0.216 | –0.362 ± 0.186 | –0.220 ± 0.274 | 0.144 ± 0.206 | 0.9974 | 0.5199 | 0.1080 |
| 2(–ΔΔCt) | 1.000 (0.716, 1.397) | 1.286 (0.969, 1.707) | 1.165 (0.762, 1.779) | 0.905 (0.661, 1.239) | |||
| Sod1 | |||||||
| ΔΔCt | 0.000 ± 0.166 | 0.021 ± 0.100 | –0.003 ± 0.174 | 0.161 ± 0.123 | 0.5189 | 0.6297 | 0.6163 |
| 2(–ΔΔCt) | 1.000 (0.773, 1.293) | 0.986 (0.846, 1.148) | 1.002 (0.765, 1.312) | 0.894 (0.741, 1.079) | |||
| Sod2 | |||||||
| ΔΔCt | 0.000 ± 0.048 | 0.060 ± 0.068 | –0.037 ± 0.054 | –0.048 ± 0.077 | 0.7103 | 0.2692 | 0.5878 |
| 2(–ΔΔCt) | 1.000 (0.927, 1.079) | 0.959 (0.865, 1.064) | 1.026 (0.944, 1.116) | 1.034 (0.919, 1.164) | |||
| Cat | |||||||
| ΔΔCt | 0.000 ± 0.051 | 0.047 ± 0.039 | –0.020 ± 0.048 | –0.038 ± 0.055 | 0.7733 | 0.2870 | 0.5078 |
| 2(–ΔΔCt) | 1.000 (0.891, 1.084) | 0.968 (0.912, 1.028) | 1.014 (0.941, 1.093) | 1.027 (0.942, 1.119) | |||
Values are means ± SEMs or fold differences relative to 10% lard (95% CIs). Two-factor ANOVA was used to identify the differences between type, percentage, and interaction (¥P < 0.05), with Tukey-Kramer post hoc conducted if significant. Abca1, ATP-binding cassette transporter 1; Apoe, apolipoprotein E; App, amyloid precursor protein; Bdnf, brain-derived neurotrophic factor; Cat, catalase; Cd36, cluster of differentiation 36/FA translocase; Insr, insulin receptor; Lrp1, LDL receptor–related protein 1; Mfsd2a, major facilitator super family domain-containing 2a; Pparα, peroxisome proliferator-activated receptor α; Pparγ, peroxisome proliferator-activated receptor γ; Glut1, glucose transporter 1; Sod1, superoxide dismutase 1; Sod2, superoxide dismutase 2; ΔΔCt, change relative to 10% Lard, with Gadph as reference gene.