Fig. 6.

Effect of GA treatment on apoptosis-related molecules in brain tissues of LPS-induced neuroinflammation: Groups of adult C57Bl/6 mice were subjected to LPS-induced neuroinflammation and treated with the indicated GA or control peptides as described in materials and methods. Equal quantity of cDNA isolated from brain tissues of mice treated with GA or CP were amplified for IL-1β, CD14, Bcl-xL, and Bcl-2 mRNA by quantitative PCR. The mRNA expression in each sample was finally determined after correction with GAPDH expression. (A) Gel electrophoresis of the PCR products GAPDH (111 bp), IL-1β (89 bp), CD14 (206 bp), Bcl-xL (84 bp), and Bcl-2 (96 bp). (B) Relative mRNA quantitation of the indicated product with respect to that of housekeeping gene GAPDH is shown. Data are average ± SD. * P < .05 with respect to vehicle- and CP-treated group. Abbreviations: GILZ, glucocorticoid induced leucine zipper; GA, glucocorticoid induced leucine zipper analog; LPS, lipopolysaccharide; CP, control peptide; Bcl-xL, B-cell lymphoma-extra large; Bcl-2, B-cell lymphoma 2; PCR, polymerase chain reaction; IL, interleukin.