Figure 2.

Structural and Functional Changes after HSP Are Reversible

(A) Experimental timeline for reversal experiments.

(B) Example traces showing cell firing following 48 hr of activity block. Spikes are demarcated with black circles.

(C) Quantification of spikes per minute throughout experiment, represented as median + IQ range. *p_{48 control–48 TTX} = 0.03, Kruskal-Wallis post-hoc Dunn; **p_{48 TTX–48 post TTX} = 0.010, Kruskal-Wallis post-hoc Dunn. Number of neurons: 48 hr TTX condition: 48 hr control = 17, 48 hr acute TTX = 20, 48 hr TTX = 26; 48 hr “post-TTX” control = 15, 48 hr post-TTX reversal = 18.

(D) Representative images from dendrites after 48 hr of HSP induction and at 48 hr post TTX. Scale bar, 5 μm.

(E) Quantification of spine sizes throughout the experiment, represented as mean ± SEM. All p values calculated using Kruskal-Wallis test, post-hoc Dunn's. ***p_{48 TTX–48 post TTX} = 4.1852 × 10⁻⁷. Number of spines (n = first number) of neurons (N = in parentheses): 48 hr control = 285 (7), 48 hr TTX = 208 (6), 48 hr “post-TTX” control = 514 (14), 48 hr post-TTX = 430 (15).