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A–C, fura‐2 ratio traces from individual HeLa cells expressing (wt)P2X4‐EGFP. Cells were stimulated with 100 μm ATP for 3 min, followed by recovery periods of 5 min (A), 10 min (B) and 30 min (C), respectively. D, ratios of second/first Ca2+ peak following repetitive ATP application in dependence on the lag between first and second ATP application (n > 62 cells for each condition).
