Figure 2.

ASOs against miR-21 Altered the Transduction of the AKT and ERK Signaling Pathway

(A) Human CRC SW620 cells were injected subcutaneously into the right flank of BALB/c nude mice (n = 10 per group). Seven days later, the plasmid of p-miR-21-ASOs (100 μg) or p-Cont (100 μg) was locally given by subcutaneous injection into the tumor tissues of nude mice four times every 3 days. Six days after the last injection, tumor tissues were collected. Protein levels of AKT, p-AKT, ERK, and p-ERK were detected using western blot, and the gray intensity value was calculated using ImageJ software. (B) Human CRC SW620 cells were transiently transfected with p-miR-21-ASOs (2.5 μg) or p-Cont (2.5 μg) in vitro. Forty-eight hours later, cells were collected, and protein levels of AKT, p-AKT, ERK, and p-ERK were detected using western blot and calculated. Representative data of three independent experiments are shown. Left: tumor tissues; right: tumor cells. *p < 0.05.