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. 2018 Oct 12;20(11):1161–1174. doi: 10.1016/j.neo.2018.09.004

Figure 1.

Figure 1

HFD feeding alters normal, noncancerous mammary ASCs in mice and humans. (A) Average body weight of FVB/N female mice fed a CD or HFD for 16 weeks (n = 5 mice/group). ASCs from CD- or HFD-fed mice were differentiated in culture into (B) adipocytes or (C) osteoblasts. Representative images of cells treated with differentiation media (DIF) compared to vehicle-treated cells (UN). Data are represented as the ratio between differentiated and undifferentiated cells (n = ASCs from 5 mice/group). (D) Cellular proliferation of isolated HFD ASCs compared to CD ASCs. Data are shown as a fold change of HFD ASC proliferation compared to CD ASC proliferation (n = ASC from 3 mice/group). (E) Cultured CD and HFD ASCs were stained for SMA using immunofluorescence. SMA expression was quantified from three images (n = 3/group). (F) Average area of collagen contracted from ASCs isolated from CD or HFD-fed mice (n = 3 mice/group). Area of collagen contraction was divided by the total number of cells present at day 6 in each gel. (G) ASCs were isolated from reduction mammoplasty tissue from women with a defined BMI. Relative expression of SMA in cultured obese (BMI >30) and lean (BMI <25) ASCs was determined using qRT-PCR (n = ASCs isolated from 3 tissue samples/group). Bars represent mean ± SD. Magnification bars = 50 μm.