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. 2018 Oct 12;20(11):1161–1174. doi: 10.1016/j.neo.2018.09.004

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© 2018 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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HFD ASCs induce an invasive phenotype in mammary tumor cells. Invasive foci into the surrounding adipose tissue were quantified from Met-1 (A) or EO771 (B) tumor cells mixed with CD or HFD ASCs (n = 4 tumors/group). Dotted lines represent the edge of the tumor surrounded by adipose tissue; arrows mark invasive foci. Tumor cells from either Met-1 (C) or EO771 (D) mixed tumors were isolated and plated on Matrigel-coated Transwells. Invading cells were quantified from three images/well in triplicate (n = 3 tumors/group). Bars represent mean ± SD. Magnification bars = 50 μm.